????? 超氧化物歧化酶對機體的氧化與抗氧化平衡起著(zhù)至關(guān)重要的作用。此酶能清除超氧陰離子自由基，保護細胞免受損傷，本試劑盒采用黃嘌呤氧化酶法(羥胺法)測定SOD活力?？蓽y血清(漿)、腦脊液、胸水、腹水、腎透析液、尿液、精液、紅細胞、白細胞、血小板、心肌培養細胞、腫瘤培養細胞、各種動(dòng)植物組織細胞、及亞細胞水平(線(xiàn)粒體、微粒體)中的SOD活力，并可檢測微生物、藥物、食品、飲料、化妝品中的SOD活力。
產(chǎn)品優(yōu)點(diǎn)如下：
1、快速簡(jiǎn)便：全程約50分鐘，可測100例左右樣本。
2、取樣量微：本法取手指或耳垂等末梢血20ul～50ul即可測紅細胞中的SOD，只需2ml靜脈血可測白細胞中的SOD及血小板中SOD，只需50mg左右組織就可測組織勻漿、胞漿中的SOD，0.2g組織可測線(xiàn)粒體及微粒體中的SOD。
3、靈敏度高：IC50=0.05g/ml，是鄰苯三酚法的18倍。
4、穩定性好：試劑盒2～8℃存放6個(gè)月有效。
5、再現性好：變異系數CV=1.7%。
6、回收試驗： X =103.3%。
7、受外界影響因素?。焊蓴_因素少，重復性強。
8、測試面廣：可測動(dòng)物血液、組織、各種體液、灌流液等、各種培養細胞、細菌、植物組織、各種水產(chǎn)以及化妝品、保健品等，效果均佳。
|
批間CV
|
批內CV
|
回收率
|
最底檢出線(xiàn)
|
檢測范圍
|
|
3.52
|
1.7
|
103
|
5.0U/ml
|
5.0-122.1 U/ml
|
本試劑盒保存期：6個(gè)月。貯存溫度：2～8℃。
1.?Kok?Poh?Loh,?Jia?Qi,?Benny?Kwong?Huat?Tan,?et?al.?Leonurine?Protects?Middle?Cerebral?Artery?Occluded?Rats?Through?Antioxidant?Effect?and?Regulation?of?Mitochondrial?Function.?Stroke,?2010,?41:2661-2668.
2.?Muxin?Wei,?Yanmin?Wu,?Dezheng?Chen,?et?al.?Changes?of?free?radicals?and?digestive?enzymes?in?saliva?in?cases?with?deficiency?in?spleen-yin?syndrome.?Journal?of?Biomedical?Research,?2010,?24(3):250-255.
3.?Zhang?Yong,?Tang?Hao-Ru,?Luo?Ya,?et?al.?Variation?in?Antioxidant?Enzyme?Activities?of?Two?Strawberry?Cultivars?with?Short-term?Low?Temperature?Stress.?World?Journal?of?Agricultural?Sciences,?2008,?4?(4):?458-462.
4.?Shao-Hua?Liu,?Ke?Ma,?Xin-Rong?Xu,?et?al.?A?single?dose?of?carbon?monoxide?intraperitoneal?administration?protects?rat?intestine?from?injury?induced?by?lipopolysaccharide.?Cell?Stress?and?Chaperones,?2010,15:717-727.
5.?Lei?Liu,?Yan?Liu,?Jie?Cui,?et?al.?Oxidative?stress?induces?gastric?submucosal?arteriolar?dysfunction?in?the?elderly.?World?J?Gastroenterol,?2013,19(48):?9439-9446.?
6.?Qifeng?Zhao,?Lan?Shao,?Xingti?Hu,?et?al.?Lipoxin?A4?Preconditioning?and?Postconditioning?Protect?Myocardial?Ischemia/Reperfusion?Injury?in?Rats.?Mediators?of?Inflammation,?Volume?2013,?Article?ID?23135,?http://dx.doi.org/10.1155/2013/231351.
7.?Gen-Xiang?Mao,?Ling-Di?Zheng,?Yong-Bao?Cao,?et?al.?Antiaging?Effect?of?Pine?Pollen?in?Human?Diploid?Fibroblasts?and?in?a?Mouse?Model?Induced?by?D-Galactose.?Oxidative?Medicine?and?Cellular?Longevity,?Volume?2012,?Article?ID?750963.?doi:10.1155/2012/?750963.
8.?Jia-Qing?Zhang,?Ming?Shen,?Cheng-Cheng?Zhu,?et?al.?3-Nitropropionic?Acid?Induces?Ovarian?Oxidative?Stress?and?Impairs?Follicle?in?Mouse.?PLoS?ONE,?2014,?9(2):?e86589.?doi:10.1371/?journal.pone.0086589.
9.?Hong-Xia?Li,?Yu?Xiao,?Ling-Ling?Cao,?et?al.?Cerebroside?C?Increases?Tolerance?to?Chilling?Injury?and?Alters?Lipid?Composition?in?Wheat?Roots.?PLoS?ONE,?2013,?8(9):?e73380.?doi:10.1371/journal.pone.0073380.
10.?Yan?Deng,?Wei?Wang,?Pingfeng?Yu,?et?al.?Comparison?of?taurine,?GABA,?Glu,?and?Asp?as?scavengers?of?malondialdehyde?in?vitro?and?in?vivo.?Nanoscale?Research?Letters,?2013,?8:190.
